Optimization of the Method of Active Ingredients Adding to the Base of Medicinal Films Depending on Certain Variables


  • L. L. Davtian Shupyk National Medical Academy of Postgraduate Education, Kyiv, Ukraine,
  • A. S. Voronkina National Pirogov Memorial Medical University, Vinnytsia, Ukraine,




metronidazole, chlorhexidine, glucosamine hydrochloride


The influence of variables of pharmaceutical factors on the technological processes of drugs manufacturing is incredibly important. Thus, in the development of a new drug in the form of medicinal films, the relevance and necessity of determining the effect of the methods of active substances adding on the effectiveness of the drug was determined.

The aim is rationalization of the method of the active pharmaceutical ingredients adding into the composition of the developed drug.

Materials and methods. As experimental samples we used medicinal films, which were made using various methods of active ingredients adding. The quality of the samples was evaluated by the antimicrobial activity against Clostridium sporogenes and Staphylococcus aureus, which was determined by the diffusion method in agar.

Results. The study of the antimicrobial activity of medicinal films with various methods of active ingredients adding showed that the adding of metronidazole as an aqueous solution increases the antimicrobial activity of the films by 21.23%, 16.89%, 28.59%, respectively, compared with films of similar composition, in which metronidazole was added as a suspension, and the remaining ingredients were added by the same way. The introduction of chlorhexidine bigluconate and glucosamine hydrochloride in the film-forming solution lastly together with the solution of metronidazole increases the antimicrobial activity by 24.67%, which is probably due to the absence of contact between thermolabile ingredients and solutions of film-forming substances having a high dissolution temperature.

Conclusions. The most rational is adding of metronidazole to the medicinal films in the form of a 0.01% aqueous solution in a mixture with the chlorhexidine bigluconate and glucosamine hydrochloride solution to the final film-forming solution.


Davtyan, L., & Voronkina, A. (2016). The study of properties of the matrix for periodontal films based on Metronidazole, Chlorhexidine and Glucosamine. Int. J. PharmTech Res., 9(2), 9–12.

Murlanova, K. S., Shirobokov, V. P., Nizhenkovska, I. V., Voronkina, A. S., Nastenko, V. B., Davtian, L. L., & Osypchuk, N. O. (2016). Doclidzhennіa antymikrobnykh vlastyvosteі parodontalnykh plivok kombinovanoho skladu [Microbiological evaluations of periodontal films containing a combination of pharmaceutical active compounds]. Ukrainskyі naukovo-medychnyі molodizhnyі zhurnal, 3(96), 68–74. [in Ukrainian].

Hurst, S., Loi, C. M., Brodfuehrer, J. & El-Kattan, A. (2007) Impact of physiological, physicochemical and biopharmaceutical factors in absorption and metabolism mechanisms on the drug oral bioavailability of rats and humans. Expert Opin Drug Metab Toxicol., 3(4), 469–89. doi: 10.1517/17425225.3.4.469.

Ashford, M. (2002) Assessment of biopharmaceutical properties. M. Aulton (Ed.), Pharmaceutics: the science of dosage form design. London: Churchill Livingstone, (P. 253–273).

Pertsev, I. M. (Ed). (2007) Farmatsevtychni ta medyko-biolohichni aspekty likiv [Pharmaceutical and medical and biological aspects of medicine]. Vinnytsia: Nova knyha. [in Ukrainian].

Pro zatverdzhennia metodychnykh vkazivok «Vyznachennia chutlyvosti mikroorhanizmiv do antybakterialnykh preparativ» vid 05 kvitnia 2007 roku №167 [On Approval of Methodical Instructions «Definition of Sensitivity of Microorganisms to Antibacterial Drugs» from April, 5, 2007, №167]. Kyiv [in Ukrainian].

Vashenko, E. S. (2008) Sravnitel'noe izuchenie stabil'nosti glyukozamina gidrokhlorida i glyukozamina sul'fata [Comparative study of the stability of glucosamine hydrochloride and glucosamine sulfate]. Razrabotka, issledovaniya i marketing novoj farmacevticheskoj produkcii. Pyatigorsk, (Vol. 63), 221–223. [in Russian].

Drozdova, A. O., Polishchuk, Yu. P. & Davtian, L. L., et al. (2012) Tekhnolohichnyi sposib vvedennia diiuchykh rechovyn do osnovy preparatu [Technological way of adding of active ingredients to the drug’s base]. Viiskova medytsyna Ukrainy, 4, 61–63. [in Ukrainian].

Korytniuk, R. S. (2005) Tekhnolohiіa ta biofarmatsevtychni aspekty likarskykh plivok antymikrobnoi dii [Technology and Biopharmaceutical Aspects of Medicinal Films of Antimicrobial Action]. Kyiv: Osnova [in Ukrainian].

How to Cite

Davtian LL, Voronkina AS. Optimization of the Method of Active Ingredients Adding to the Base of Medicinal Films Depending on Certain Variables. Current issues in pharmacy and medicine: science and practice [Internet]. 2018Feb.16 [cited 2024May24];(1). Available from: http://pharmed.zsmu.edu.ua/article/view/123719



Pharmaceutical manufacturing