The study of an acute toxicity of extracts from Veronica teucrium L. and from Veronica longifolia L.
DOI:
https://doi.org/10.14739/2409-2932.2018.1.123683Keywords:
Veronica, acute toxicity tests, plant extractsAbstract
The aim of our work were to study the acute toxicity of extracts obtained from herb, leaves, flowers of V. teucrium L. and from herb of V. longifolia L.
Materials and methods. The study of the acute toxicity of extracts obtained from herb (Vt-h-w), leaves (Vt-l-chl), flowers (Vt-fl-chl) of V. teucrium L. and herb of V. longifolia L. (Vl-h-sp) was carried out in accordance with International and domestic requirements for humane attitude towards animals. The study was carried out with white, nonlinear, sexually mature, standardized on the physiological and biochemical parameters male mice, divided into 5 groups in 6 animals in each. The investigated extracts were administered intragastrically at a dose of 5000 mg/kg. The assessment of acute toxicity was carried out according to the general condition, dynamics of body mass, calculation of fatal cases of experimental animals, hematological and biochemical parameters. For confirmation of extracts non-toxicity, the histological study of internal organs samples was performed by 14 days from the beginning of the experiment. The class of toxicity was determined by the classification of K. K. Sidorov.
Results. The intragastric administration of extracts from V. teucrium L. and from V. longifolia L. in a dose of 5000 mg/kg is not accompanied by the death of experimental animals and changed in their behavior, which indicates the absence of significant toxic effects of substances. The general condition, dynamics of body mass and other parameters were satisfactory and didn’t differ from parameters of intact animals. The investigated extracts didn’t influence to hematological and biochemical parameters of intact animals, and were within the limits of parameters of intact animals. Pathohistological changes in the structure of internal organs (myocardium, lungs, stomach, liver and kidneys) under the influence of the investigated substances were not detected.
Conclusions. Significantly cases of an intoxication of animals were not registered, the general condition, dynamics of body mass and other parameters were satisfactory and didn’t differ from parameters of intact animals. Hematological and biochemical parameters were within the limits of parameters of intact animals. Pathohistological changes in the structure of internal organs were not detected. According to the classification of K. K. Sidorov extracts are classified as the IV class of toxicity: low toxicity substances (LD50 = 500–5000 mg/kg).
References
Kashpur, N. V., Goryacha, O. V., Ilyina, T. V., Kovalyova, A. M., & Volyanskiy, A. J. (2011). Doslidzhennia toksychnosti ta alerhizuiuchoi dii lipofilnykh fraktsii predstavnykiv rodu pidmarennyk [The study of toxicity and allergenic activity of lipophilic fractions of Galium L. genus representatives]. Ukrainskyi zhurnal klinichnoi i laboratornoi medytsyny, 6(4), 200–204. [in Ukrainian].
Mosyakin, S. L., & Fedoronchuk, M. M. (1999). Vascular Plants of Ukraine. A nomenclatural checklist. Kyiv. [in English].
Osmachko, A. P., & Kovalova, A. M. (2016). Perspektyvy vykorystannia roslyn rodiv Veronica, Artemisia ta Lamium yak antybakterialnykh zasobiv [Prospects for using plants of genera Veronica, Artemisia and Lamium as antibacterial agents] Liky – liudyni. Suchasni problemy farmakoterapii i pryznachennia likarskykh zasobiv. Proceedings of XXXІІІ the All-Ukrainian Scientific and Practical Conference with international participation. Kharkiv. [in Ukrainian].
Mocan, A., Vodnar, D. C., Vlase, L., Crișan, O., Gheldiu, A. M., & Crișan, G. (2015). Phytochemical characterization of Veronica officinalis L., V. teucrium L. and V. orchidea Crantz from Romania and their antioxidant and antimicrobial properties. International Journal of Molecular Sciences, 16(9), 21109–21127. doi: 10.3390/ijms160921109.
Beara, I., Zivkovic, J., Lesjak, M., Ristic, J., Savikin, K., Maksimovic, Z., & Jankovic, T. (2015). Phenolic profile and anti-inflammatory activity of three Veronica species. Industrial Crops and Products, 63, 276–280. doi: 10.1016/j.indcrop.2014.09.034.
Osmachko, A. P., Kovalova, A. M., Iliina, T. V., Kashpur, N. V., Komisarenko, A. M., Koshovyi, O. M., et al. (patentee) (2017). Patent Ukrainy na korysnu model MPK (2017.01) А61К 36/38, А61К 127/00, А61К 133/00, А61Р 37/02. №u201701098. Sposib oderzhannia kompleksu polisakharydiv z imunomoduliuiuchoiu aktyvnistiu [Patent of Ukraine to the utility model А61К 36/38, А61К 127/00, А61К 133/00, А61Р 37/02. Method for obtaining of a polysaccharides complex with immunomodulatory activity]. Biulleten, 12. [in Ukrainian].
(1986). European Convention for the protection of vertebrate animals used for experimental and other scientific purposes. Council of Europe, Strasbourg.
Zakon Ukrainy «Pro zakhyst tvaryn vid zhorstokoho povodzhennia» vid 15 hrudnia 2009 roku №1759-VI (1759-17) [The Law of Ukraine «On Protection of Animals from Cruel Treatment» of December 15, 2009, №1759-VI (1759-17)]. Vidomosti Verkhovnoi Rady Ukrainy, 9. [in Ukrainian].
Reznikov, O. H., Solovyov, A. I., Dobrelia, N. V., & Stefanov, O. V. (2006). Bioetychna ekspertyza doklinichnykh ta inshykh naukovykh doslidzhen, shcho vykonuiutsia na tvarynakh. [Bioethical expertise of preclinical and other scientific researches, what carried out on animals: methodical recommendation]. Kyiv. [in Ukrainian].
Stefanov, O. V. (Ed) (2001). Doklinichni doslidzhennia likarskykh zasobiv [Preclinical research of medicines]. Kyiv: Avitsena. [in Ukrainian].
Jaijoy, K., Baths, S., Piyabhan, P., Lerdvuthisopon, N., Boonraeng, S., Khonsung, P., et al. (2011). Acute and subchronic toxicity study of the water extract from the fruits of the Piper Chaba Hunter in the rat. International journal of applied research in natural products, 3(4), 29–35.
Jothy, S., Zakaria, Z., Chen, Y., Lau, Y., Latha, L., & Sasidharan, S. (2011). Acute Oral Toxicity of Methanolic Seed Extract of Cassia fistula in Mice. Molecules, 16(6), 5268–5282. doi: 10.3390/molecules16065268.
Menshikova, V. V. (Ed.) (1987). Laboratornyye metody issledovaniya v klinike [Laboratory methods of research in the clinic]. Moscow: Medicina. [in Russian].
D'yakon, A. V., Khrykina, I. S., Khegaj, A. A., D'yachenko, I. A., Murashev, A. N., & Ivashev, M. N. (2013). Metod zabora krovi u zhivotnykh. Mezhdunarodnyj zhurnal prikladnykh i fundamental'nykh issledovanij № 11-2 [Method of blood collection from animals. International Journal of Applied and Fundamental Research № 11-2]. Moscow: Akademiya Yestestvoznaniya. [in Russian].
Sidorov, K. K. (1979). O klassifikacii toksichnosti yadov pri parenteral'nykh sposobakh vvedeniya [On the classification of the toxicity of poisons with parenteral routes of administration]. Toksikologiya novykh promyshlennykh, khimicheskikh veshchestv, 13, 47–51. [in Russian].
Downloads
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access)
