Investigation of antiulcer activity of the thick extract of immature walnut fruits on the model of diclofenac-reduced gastric ulcer of rats
Keywords:walnut, plant extracts, anti-ulcer agents, peptic ulcer, plant preparations
AbstractTopicality. It is known from the literature that the walnut composition includes tannins, derivatives of ellagic acid, catechins, derivatives of dicarboxylated acids, nucleic acids, amino acids that independently of one another have antioxidant, hypoglycemic, antiproliferative, antimicrobial, anti-inflammatory and many other beneficial effects in the human body. However, there is no experimental data on the effect of the extract of thick water-alcohol immature walnut fruit (ETWA) on gastric ulcer of rats caused by the introduction of diclofenac.
Aim. To study the gastroprotective effect of ETWA on the model of diclofenac-reduced ulcer of rats.
Materials and methods. Pharmacological study of antiulcer activity of ETWA was performed on the model of diclofenac-reduced gastric ulcer, reproducible in rats in accordance with the methodological recommendations of the State Pharmacological Center Ukraine. Biochemical studies of blood plasma were carried out using standard test kits of NIP "Filisit-Diagnostika" (Ukraine) according to generally accepted methods. The results of the study were processed using a statistical package of the program "STATISTICA® for Windows 7.0" (StatSoft Inc.).
Results and discussion. Based on the results of the study of the gastroprotective effect of ETWA at a dose of 25 mg/kg, at autopsy and stomach examination, ulcers in this group of animals were found to be significantly smaller in size than in the group of model pathology (p˃0.05). In the group of animals taking ETWA at a dose of 25 mg/kg, the indicator of antiulcer activity was 76.67%. The application of the extract had a pronounced normalizing effect on the state of the biochemical parameters of blood serum of rats in relation to the group of model pathogens.
Conclusions. Introduction in the treatment-and-prophylactic regimen of the studied ETWA at a dose of 25 mg/kg, provides antiulcer activity in the model of acute gastric ulcer caused by diclofenac administration. In addition, the activity of the studied ETWA at a dose of 25 mg/kg is not inferior in activity to the reference preparation.
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