Characteristic of the chemical composition of Artemisia pontica L

Materials and methods. Research material (Artemisia pontica L. herb) was collected in August 2019 in the Zaporizhzhia region, Ukraine. The tincture was obtained by maceration. So, the tincture was being extracted with methyl alcohol at room temperature for 10 days. The tincture was extracted with methyl alcohol at room temperature for 10 days according to the method of preparing tinctures. The study of the chemical composition of tincture of Artemisia pontica L. was carried out using gas chromatograph Agilent 7890B GC System (Agilent, Santa Clara, CA, USA) with mass spectrometric detector Agilent 5977 BGC/MSD (Agilent, Santa Clara, CA, USA) and chromatographic column DB-5ms (30 m × 250 mkm × 0.25 mkm).

It is used in medicine as an antiseptic for the oral cavity (Maraslavin, Bulgaria). Research data revealed the presence of antiinflammatory, analgesic, wound healing, antifungal, and antimicrobial effects [3,4].
Artemisia pontica L. was previously cultivated as a spicyaromatic plant in Western Europe and North America. Although Artemisia pontica L. is grown as an ornamental plant in gardens, it can also be used as feed for small livestock, horses, and camels [5].
Despite the potential perspective of using this plant in medicine, the chemical composition of Artemisia pontica L. populations that grow in Ukraine is not well investigated. Before the research of the chemical composition of the herb Artemisia pontica L. was conducted.

Aim
To determine the qualitative and quantitative characteristics of biologically active compounds of Artemisia pontica L. Definition prospects of medicinal herbal raw materials in the treatment of diseases.

Materials and methods
Plant materials. Research material (Artemisia pontica L. herb) was collected in August 2019 in the Zaporizhzhia region. Herbarium samples were deposited at the herbarium of the Faculty of Pharmacy of Zaporizhzhia State Medical University.
Extraction. According to the general method of preparing tinctures, the tincture was obtained by maceration. The tincture was being extracted with methyl alcohol at room temperature for 10 days [13]. Then 0.1 ml of tincture was placed in a 1 ml micro flask and was brought with methanol to 0.5 ml.
Equipment. The completeness of the reactions and the in dividuality of the resulting compounds were controlled by the gas chromatograph Agilent 7890B with a 5977B mass spectrometry detector. The column was DB5ms 30 m × 250 μm × 0.25 μm with length. The gascarrier speed (helium) was 1.6 ml/min. Injection volume was 0.5 μl. The separation of the flow was 1:50. The temperature of the sampling unit was 230 °C → 12 °C/s → 275 °C. Thermostat temperature: programmable, 240 °C (1minute ISSN 2306-8094 Characteristic of the chemical composition of Artemisia pontica L. delay) → 5 °C/min → 280 °C (1minute delay). The total time of examination were 10 min. Temperature of interface GS/MS -280 °C; ion sources -230 °C; quadrupole mass analyzer -150 °C. Type of ionization: EI with an electron energy of 70 eV. The range of mass numbers that was scanned: 30-500 m/z.
The results of the study are in Table 1.
Such components as nhexadecanoic acid with a retention time of 16.27 min and 9,12,15(Z,Z,Z)octadecatrienoic acid (RT = 17.917 min) were identified on chromatogram of the components of Artemisia pontica L. (Fig. 1).

Discussion
The populations of Artemisia pontica L. that grow on the terri tory of Ukraine differ significantly from the populations of other countries in chemical composition. The main active ingredients of the Kazakhstan fraction are flavonoids 7Omethyland 4′,7diOmethylesters of apigenin [14], while the Ukrainian fraction is dominated by fatty acids. Regarding the study of the component composition of water extracts of other Arte-  [15].
It should be noted that the terpenoid fraction of Artemisia pontica L. was significantly different in chemical composition from the other representatives of the genus Artemisia L. For example, A. absinthium L. had the highest βmyrcene content, A. austriaca Jacq. had camphor [9], and A. frigida Willd. had 1,8cineole and camphor, A. argyrophylla contained artemisia ketone and artemisia alcohol acetate [19].
The literature review illustrated that nhexadecanoic acid (palmitic acid) was the most common fatty acid in nature, which was a part of the glycerides of most animal fats, ve getable oils, and waxes. It had stimulated antiinflammatory processes in human immune cells through TLR4 receptors [20], it had antitumor and antidiabetic activity [21] and it also had antiinflammatory effects [22]. 9,12,15(Z,Z,Z)octadecatrienoic acid was an isomer of 5,9,12(Z,Z,Z)octadecatrienoic acid that was better known as pinolenic acid. It had been established that pinolenic acid can affect immune processes [23], and was a dual FFA1/FFA4 agonist with potential effect against metabolic diseases such as insulin resistance and type 2 diabetes [24].
There was also experimental data on the presence of choles terollowering effect on the LDL receptor activity of human hepatoma HepG2 cells [25]. Investigations of pinolenic acid as an inhibitor of human breast cancer MDAMB231 cell metastasis in vitro may be perspective [26].
In addition, it was necessary to underline the presence of neophytadiene that has an antiinflammatory effect [28].
Considering the given all the above the herb Artemisia pontica L. can be recommended for further studies due to its antiinflammatory, antidiabetic and antitumor effects.
Prospects for further research. The herb of Artemisia pontica L. is a source of biologically active substances that have antiinflammatory, antidiabetic and antitumor effects.

Funding
The research is an integral part of the joint complex work of Department of Pharmacognosy, Pharmacology and Botany of Zaporizhzhia State Medical University.