A study of actoprotective activity of new 3-(thiophen-2-ylmethyl)- 1H-1,2,4-triazole-5-thiol derivatives

The issue of fatigue is quite topical for the modern humanity. In order to work harder and earn as much money as possible, a person takes various stimulants, which have a number of side effects. This problem is especially serioous in Asian countries. To prevent such complications, scientists are trying to invent actoprotectors that would have minimal side effects. 1,2,4-triazole derivatives have proven themselves well as new substances with different spectrum of pharmacological activity.

Scientists around the world are competing for the leadership in creating new drugs that reduce fatigue and help athletes increase their speed and improve performance.
Pharmacological activity was studied with the method of forced swimming [12]. Loads (10 % of rats' weight) were attached to the tail bases of the animals. After immersing the animals in water for 10 seconds, the swimming time of the laboratory rats before exhaustion was measured (in seconds). Rats were immersed one by one in a large container (water temperature 24-27 °C) with a water level higher than 60 cm. The study compounds, as well as the reference standard -Riboxin ® (manufactured by Kyiv Vitamin Plant) were administered orally 20 minutes prior to the immersion of animals at a dose of 100 mg/kg. For comparison, we also used a control group of animals that received saline 20 minutes prior to the immersion.
Statistical results were calculated using Kolmogorov-Smirnov test and Shapiro-Wilk test.

Results
As a result, the actoprotective activity of 22 new compounds was investigated.
Compounds Ia, IIb, IIh, have been found to have an actoprotective effect. Compound Ia surpasses the comparison drug.
Some conclusions have been made regarding "structureactoprotective activity" dependence.
It should be noted that compounds IIb, IIh, IIj reduce the fatigue of the studied rats and have an actoprotective effect of 20.31 %, 24.27 % and 16.24 %, respectively. But despite the positive result, these compounds do not surpass the comparison drug riboxin (Fig. 2).

Conclusions
As a result, the actoprotective activity of 22 new compounds was investigated.
Compounds Ia, IIb, IIh have been found to have an actoprotective effect. Compound Ia surpasses the comparison drug.

Funding
The research is carried out in the framework of the research activities of Zaporizhzhia State Medical University "Synthesis, physicochemical   Table 4.